Automatic Kjeldahl determination of protein content advantages

The Kjeldahl method is semi-micro and automatic. The operation of the semi-micro Kjeldahl method is relatively tedious, and the automatic method is relatively efficient. The Kjeldahl method includes steps such as digestion, distillation, titration, and calculation of results, and three kinds of national standards Kjeldahl method for determination of protein content. The automatic Kjeldahl determination method can be set according to the requirements of the test method. It is easier to meet the accuracy and repeatability requirements than the semi-micro Kjeldahl method. When the sample size is large, it can be greatly improved. Work efficiency.

If the reaction temperature is too low, the sample will be incompletely digested, leading to low detection results. If the reaction temperature is too high, the nitrogen content of the decomposed sample will be lost, which will also lead to a low detection result. For the same soybean sample, use automatic Kjeldahl nitrogen determination method, semi-micro Kjeldahl method and non-standard semi-micro Kjeldahl method. (The sample is not standardized during digestion, use 2kW electric furnace does not pad asbestos network , directly heat the Kjeldahl nitrogen bottle, the sample digestion temperature is too high), and conduct 5 parallel comparison test.

Automatic Kjeldahl determination of soybean protein content is highly automated, standard deviation is minimal, and repeatability is good; use Kjeldahl method to determine protein, 2kW electric furnace does not pad asbestos network, direct heating leads to high digestion temperature, The loss of nitrogen is more, and the measured value is about 30% to 60% of the actual value. In the process of sample digestion, special attention should be paid to the standard operation to control the size of the firepower, otherwise it will lead to the loss of nitrogen, leading to a severely low value of the measured value of the protein. It is advisable to use an automated Kjeldahl apparatus for digesting samples, and to determine the recoveries of N-acetamide or tryptophan by inspection tests to determine the conditions of the instrument to ensure the accuracy and repeatability of the measurement results. Sex. If the use of electric furnace for artificial digestion, we must control the reaction temperature, when heated pad asbestos network to ensure uniform heating, and at the same time do a good job of inspection and test.

The use of automatic Kjeldahl determination of protein content, can effectively prevent digestion, distillation and other processes due to human error in the experimental results, ensuring the accuracy and reliability of the test results. In strict accordance with the operating specifications and test methods required to operate in the sample digestion process to control the reaction temperature, can reduce the error of the test results.

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